Development of cell surface saccharides on embryonic pancreatic cells
نویسندگان
چکیده
منابع مشابه
Development of cell surface saccharides on embryonic pancreatic cells
Using a battery of seven lectin-ferritin conjugates as probes for cell surface glycoconjugates, we have studied the pattern of plasmalemmal differentiation of cells in the embryonic rat pancreas from day 15 in utero to the early postpartum stage. Our results indicate that differentiation of plasmalemmal glycoconjugates on acinar, endocrine, and centroacinar cells is temporally correlated with d...
متن کاملDistribution of cell surface saccharides on pancreatic cells
We describe here a simple, general procedure for the purification of a variety of lectins, and for the preparation of lectin-ferritin conjugates of defined molar composition and binding properties to be used as probes for cell surface saccharides. The technique uses a "universal" affinity column for lectins and their conjugates, which consists of hog sulfated gastric mucin glycopeptides covalen...
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The surface saccharide composition of collagenase-dispersed pancreatic cells from adult guinea pig and rat glands was examined by using eight lectins and their ferritin conjugates: Concanavalin A (ConA); Lens culinaris (LCL); Lotus tetragonolobus (LTL); Ricinus communis agglutinins I and II (RCA I, RCA II); Soybean agglutinin (SBA); Ulex europeus lectin (UEL); and wheat germ agglutinin (WGA). B...
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15 صفحه اولCell surface properties of amphibian embryonic cells.
Gastrula and neurula embryos of Cynops pyrrhogaster were dissociated into cell suspensions with ethylenediaminetetraacetic acid (EDTA), trypsin, and solution. The cells were cultured in Niu-Twitty's balanced salt solution and their aggregates were examined histologically. The results indicated that the capacity of amphibian embryonic cells for aggregate formation, sorting out, and notochord dif...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1980
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.86.1.96